Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 4959-4962.doi: 10.3969/j.issn. 2095-4344.2012.27. 004

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Co-culture of rabbit bone marrow mesenchymal stem cells and human amniotic membrane

Zhang Zhi-jin, Guo Lin, Zhao De-wei   

  1. Department of Orthopedics, Zhongshan Hospital Affiliated to Dalian Medical University, Dalian 116001, Liaoning Province, China
  • Received:2011-12-03 Revised:2012-01-17 Online:2012-07-01 Published:2013-11-01
  • Contact: Guo Lin, Master, Professor, Department of Orthopedics, Zhongshan Hospital Affiliated to Dalian Medical University, Dalian 116001, Liaoning Province, China glds2010@163.com
  • About author:Zhang Zhi-jin, Studying for master’s degree, Department of Orthopedics, Zhongshan Hospital Affiliated to Dalian Medical University, Dalian 116001, Liaoning Province, China zzj20030606@ qq.com

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Abstract:

BACKGROUND: In recent years, great progress has been made in repair of cartilage defects using tissue engineering technique. Bone marrow mesenchymal stem cells (BMSCs) as common seed cells have been widely used in the tissue engineering of cartilage tissue.
OBJECTIVE: Human amniotic membrane was used as cell scaffold to load rabbit BMSCs and the biocompatibility of human amniotic membrane was observed.
METHODS: Rabbit BMSCs were inoculated onto human acellular amnion. After co-culture, cell morphology was observed. The proliferation of BMSCs was investigated by MTT assay, and the histocompatibility of human amniotic membrane was evaluated. Cell survival and apoptosis was determined by 4’, 6-diamidino-2-phenylindole.
RESULTS AND CONCLUTION: BMSCs grew well on the surface of human amniotic membrane and cell viability increased. DAPI staining results showed that only a small number of cells were apoptotic. These findings suggest that human amniotic membrane is a scaffold material with good biocompatibility and can promote the proliferation of BMSCs.

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